Journal: Journal of Visualized Experiments : JoVE

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

doi: 10.3791/3791

Figure Lengend Snippet:

Article Snippet: However, customized antibody microarray manufacture can be readily done from a service provider such as Serome Biosciences Inc. For a robust result, we recommend a non-contact microarrayer, such as the Scienion sciFLEXARRAYER ultra low volume non-contact microarrayer which was used in our antibody microarray manufacture.

Techniques: Microarray, Staining, Incubation, Protease Inhibitor

Journal: PLoS ONE

Article Title: Identification of the Avian RBP7 Gene as a New Adipose-Specific Gene and RBP7 Promoter-Driven GFP Expression in Adipose Tissue of Transgenic Quail

doi: 10.1371/journal.pone.0124768

Figure Lengend Snippet: A) Relative RBP7 mRNA expression values from chicken cDNA microarray (n = 3, pooled from three chickens for each tissue). B) Measurement of RBP7 mRNA expression in chicken tissues by qPCR (n = 4) which is normalized to β-actin as a housekeeping gene. S-Fat: subcutaneous fat, A-Fat: abdominal fat, T-Mus: thigh muscle, P-Mus: pectoralis major muscle.

Article Snippet: An avian RBP7 polyclonal antibody was raised against an immunogen including amino acids 73–83 residues of chicken RBP7 protein in the rabbit by a custom antibody service (AbClon, Seoul, Korea).

Techniques: Expressing, Microarray

Journal: PLoS ONE

Article Title: Identification of the Avian RBP7 Gene as a New Adipose-Specific Gene and RBP7 Promoter-Driven GFP Expression in Adipose Tissue of Transgenic Quail

doi: 10.1371/journal.pone.0124768

Figure Lengend Snippet: A) Comparison of RBP7 amino acid sequences of chicken (GenBank accession number XP_417606.4), turkey (XP_003212313.1), quail (corresponding to KP026122), human (NP443192.1) and mouse (NP071303.1). An epitope sequence detected by a custom RBP7 antibody is underlined from residue 73 to 83. B) Western blot analysis of RBP7 protein expression in chicken, quail and turkey tissues, respectively. α-tubulin was used as a loading control. SF: subcutaneous fat, AF: abdominal fat, TM: thigh muscle, PM: pectoralis major muscle, H: heart, Lu: lung, Li: liver, Ki: kidney, Sp: spleen, Br: brain, Int: intestine.

Article Snippet: An avian RBP7 polyclonal antibody was raised against an immunogen including amino acids 73–83 residues of chicken RBP7 protein in the rabbit by a custom antibody service (AbClon, Seoul, Korea).

Techniques: Comparison, Sequencing, Residue, Western Blot, Expressing, Control

Journal: PLoS ONE

Article Title: Identification of the Avian RBP7 Gene as a New Adipose-Specific Gene and RBP7 Promoter-Driven GFP Expression in Adipose Tissue of Transgenic Quail

doi: 10.1371/journal.pone.0124768

Figure Lengend Snippet: A) RBP7 promoter with regulatory elements interacting with major transcription factors in adipose tissue. B) Schematic representation of the pLT-RBP7p3k-eGFP vector. Restriction sites, ClaI and PacI are depicted. LTR, long terminal repeat; ψ, packaging signal; RRE, rev-response element; cPPT, central polypurine tract of HIV-1; eGFP, enhanced green fluorescent protein; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element. The arrows represent the location of forward and reverse primers used for genotyping PCR. C) Genotyping PCR of transgenic and wild-type quail using the two sets of primers depicted in the figure of B). WT, wild-type quail; +: pLT-RBP7p3k-eGFP plasmid DNA (positive control).

Article Snippet: An avian RBP7 polyclonal antibody was raised against an immunogen including amino acids 73–83 residues of chicken RBP7 protein in the rabbit by a custom antibody service (AbClon, Seoul, Korea).

Techniques: Plasmid Preparation, Virus, Transgenic Assay, Positive Control

Journal: PLoS ONE

Article Title: Identification of the Avian RBP7 Gene as a New Adipose-Specific Gene and RBP7 Promoter-Driven GFP Expression in Adipose Tissue of Transgenic Quail

doi: 10.1371/journal.pone.0124768

Figure Lengend Snippet: A) Quantification of RBP7, DLK1, FABP4, and PPARγ mRNA expression in SV and FC fractions from chicken adipose tissue by qPCR (n = 4). β-actin expression was used as a normalization control. B) qPCR analysis of SV and FC fractions from adipose tissue of selected transgenic G2 quail for mRNA expression of GFP, SCD1, FABP4, and RBP7 (n = 3). Ribosomal protein S13 ( RPS13 ) was selected as a reference gene. C) Phase contrast and GFP images of primary preadipocytes and adipocytes from K5 and K7 transgenic G2 quail.

Article Snippet: An avian RBP7 polyclonal antibody was raised against an immunogen including amino acids 73–83 residues of chicken RBP7 protein in the rabbit by a custom antibody service (AbClon, Seoul, Korea).

Techniques: Expressing, Control, Transgenic Assay